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目的观察ki67在鼻咽癌组织的表达,从量化角度阐明其在鼻咽癌组织中表达的病理学意义。方法常规石蜡包埋、HE切片确诊,用免疫组化SP法检测ki67在鼻咽癌细胞核中的定位和表达,用Leica Q500MC图像分析系统对其表达强度进行定量分析,并用阳性单位(positive unit,PU)反映其表达强度。结果 ki67在鼻咽癌组织中的阳性表达主要定位在细胞核,ki67阳性细胞为核境界清晰的棕色、棕褐色颗粒沉着细胞。本研究显示ki67表达与鼻咽癌患者有无颈淋巴结转移、治疗方式不具有相关性,经统计学检验ki67与鼻咽癌患者有无远处转移、生存期及临床分期有相关性,数据具有统计学意义。有远处转移的PU值(21.00±5.95)高于无远处转移者(16.82±4.55),t=-3.069,P=0.004,两者的数据具有统计学意义;我们发现不同生存期患者的表达量均有不同,随着生存期的延长,ki67的阳性表达强度逐渐减弱。生存期3年以下PU值(24.39±4.50)高于生存期3～5年的(17.69±2.61),生存期3～5年的PU值高于生存期超过5年的(15.10±3.51),三组之间PU值差异具有统计学意义(F=45.484,P=0.000)。随着临床分期I、II、III、IV期的顺序,鼻咽癌患者的ki67的阳性表达强度逐渐增强,PU值逐渐加大,组间差异具有统计学意义(t=7.271,P=0.002)。说明ki67表达阳性者多出现在NPC的晚期。结论 ki67蛋白表达程度对于判断鼻咽癌患者是否存在远处转移和患者生存时间具有重要价值,表明Ki67可作为判定鼻咽癌的恶性程度和预后的指标之一。
Objective To observe the expression of ki67 in nasopharyngeal carcinoma and to elucidate its pathological significance in nasopharyngeal carcinoma from the quantitative point of view. Methods The paraffin-embedded sections were confirmed by HE chip. The localization and expression of ki67 in the nucleus of nasopharyngeal carcinoma were detected by immunohistochemical SP method. The expression intensity of ki67 was quantitatively analyzed by Leica Q500MC image analysis system. Positive cells (positive unit, PU) reflects its expression intensity. Results The positive expression of ki67 in nasopharyngeal carcinoma mainly localized in the nucleus. Ki67-positive cells were clear brown and tan granule cells in the nucleus. This study showed that ki67 expression and nasopharyngeal carcinoma patients with or without cervical lymph node metastasis, the treatment is not related to the statistical test ki67 and nasopharyngeal carcinoma patients with or without distant metastasis, survival and clinical stage correlation, the data have Statistical significance. The PU value with distant metastasis (21.00 ± 5.95) was higher than that without distant metastasis (16.82 ± 4.55), t = -3.069, P = 0.004, the data of both were statistically significant; we found that the patients with different survival The expression levels were different, with the extension of survival, ki67 positive expression intensity gradually weakened. The PU value of survival time of 3 years or less (24.39 ± 4.50) was higher than that of survival time of 3 ~ 5 years (17.69 ± 2.61), the PU value of survival time of 3 ~ 5 years was higher than that of survival time of more than 5 years (15.10 ± 3.51) There was a significant difference in PU values among the three groups (F = 45.484, P = 0.000). With the sequence of stage I, II, III and IV, the positive expression of ki67 in nasopharyngeal carcinoma patients gradually increased, and the PU value gradually increased. The difference between the two groups was statistically significant (t = 7.271, P = 0.002) . The positive expression of ki67 appear more in the advanced stage of NPC. Conclusion The expression of ki67 protein is of great value in judging the existence of distant metastasis and survival time in patients with nasopharyngeal carcinoma, indicating that Ki67 can be used as one of the indicators to determine the degree of malignancy and prognosis of NPC.