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作者观察中国田鼠肺纤维细胞V79在次黄嘌呤-鸟嘌呤磷酸核糖转移酶(HPRT)位点上辐射诱发突变的程度,来评价在急性低氧情况下WR-1065抗突变的效果.方法:V79-B310H在37℃100mm陪替氏培养皿中(α-MEM-10培养基加10%胎牛血清)生长,使用前,培养的细胞在α-MEM-10培养基(含有5×10~(-5)mol次黄嘌呤、3.2×10~(-6)mol氨基喋呤及5×10~(-6)mol胸腺核苷,〔HAT〕)中生长24h,以减少HPRT突变的自然本底.使用前,细胞从HAT培养基移到常规培养基中生长至少6天.当需要时,在1mL含10~6细胞的玻璃安瓿内通入2min湿的95%N_2和5%CO_2灭菌混合气体造成急性低氧状态.通气前加1
To observe the effect of WR-1065 anti-mutagenesis on the hypoxia-induced anti-mutagenicity of V79 in hypoxia-guanine phosphoribosyltransferase (HPRT) -B310H was grown in a 100 mm petri dish (α-MEM-10 medium supplemented with 10% fetal bovine serum) at 37 ° C. Cells were cultured in α-MEM-10 medium containing 5 × 10 ~ -5) mol hypoxanthine, 3.2 × 10 ~ (-6) mol aminopterin and 5 × 10 -6 mol thymidine ([HAT]) for 24 h to reduce the natural background of HPRT mutation Prior to use, cells were transferred from HAT medium to normal medium for at least 6 days. When required, 1 mL of wet 95% N 2 and 5% CO 2 sterilization mix was introduced into 1 mL glass ampoules containing 10 to 6 cells Gas causes acute hypoxia