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目的 探讨大鼠矽肺模型对绵羊红细胞 (SRBC)抗原的免疫应答。方法 矽肺模型大鼠分别通过气道、腹腔给予SRBC ,观察其血清溶血素、血清与肺泡灌洗液特异性IgG水平的动态变化、皮肤迟发型变态反应 (DTH)以及肺引流淋巴结、肺泡灌洗液细胞总数和分类计数。结果 (1 )第一次抗原免疫后第 7、1 2、2 0、2 5、32天 ,第二次抗原免疫后第 5、1 2、1 5天 ,矽肺气道免疫组大鼠血清溶血素半数溶血指数 (HC50 )分别为 47.4± 1 .0、52 .2± 4 .6、31 .1± 1 1 .9、43 .8± 3 .5、33 .6± 1 6 .8、49.0± 2 .3、92 .9± 2 0 .2、87.7± 5 .2 ,高于正常对照组 (分别为 40 .4± 1 0 .6、2 .8± 2 .5、0 .8± 0 .6、6 .6± 5 .8、1 .4±0 .1、36 .5± 1 6 .5、53 .0± 33 .2、2 .6± 2 .2 ) ,差异均有显著性 (P <0 .0 1 )。 (2 )第一次抗原免疫后第 1 2、2 0、2 5、32天 ,第二次抗原免疫后第 5、1 2、1 5、2 7天 ,矽肺气道免疫组大鼠血清特异性IgG(A4 90 nm值 )分别为 1 .67± 0 .1 9、1 .98± 0 .36、1 .1 2± 0 .50、1 .38± 0 .30、2 .75± 0 .1 5、2 .60± 0 .2 8、2 .86± 0 .1 0、2 .50±0 .2 0 ,高于正常对照组 (分别为 0 .59± 0 .30、0 .56± 0 .2 1、0 .2 1± 0 .1 6、0 .2 2± 0 .1 0、0 .81± 0 .2 5、0
Objective To investigate the immune response of rat silicosis model to sheep erythrocyte (SRBC) antigen. Methods Silicosis model rats were given airway and intraperitoneal administration of SRBC respectively to observe the dynamic change of serum hemolysin, serum and BALF-specific IgG level, delayed skin allergy (DTH) and pulmonary draining lymph node, alveolar lavage The total number of liquid cells and the classification of the count. Results (1) Serum hemolysis of the silicotic airway immunized rats on days 1, 2, 2, 0, 25, 32 and 5, The half-value hemolytic index (HC50) were 47.4 ± 1 .0,52 .2 ± 4 .6,31 .1 ± 1 1 .9,43 .8 ± 3 .5,33 .6 ± 1 6 .8,49.0 ± 2 .3,92 .9 ± 20.2,87.7 ± 5.2, higher than the control group (40.4 ± 1.06, 8.2 ± 2.5, 0.8 ± 0 .6,6 .6 ± 5 .8,1 .4 ± 0 .1,36 .5 ± 16.5,53 .0 ± 33.2, 2.6 ± 2.2), the differences were significant (P <0. 01). (2) After the first immunization, the serum levels of serum in the silicotic pulmonary airway injury group were significantly higher than those in the control group on days 1, 2, day 2, day 2, day 2, day 2, day 2, day 2 and day 2, Sex IgG (A490 nm values) were 1.67 ± 0.19, 1.98 ± 0.36, 1.12 ± 0.50, 1.38 ± 0.30 and 2.75 ± 0, respectively. 1 5,2 .60 ± 0 .2 8,2 .86 ± 0 .1 0,2 .50 ± 0 .2 0, higher than the normal control group (0 .59 ± 0 .30,0 .56 ± 0 .2 1,0 .2 1 ± 0 .1 6,0 .2 2 ± 0 .1 0,0 .81 ± 0 .2 5,0