论文部分内容阅读
A new indirect inhibitive immunoassay using surface plasmon resonance(SPR)coupled with molecularly imprinted polymer(MIP)was developed.A sulfamethoxazole(SMX)MIP coating capillary was produced and used as an in-tube solid phase extraction(SPE)device.The MIP coating formed a nanometer film on the inner wall of the capillary.The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner.The calibration curve was generated by linear fit over the range of0.04-10.00 ng/mL.The limit of detection was 0.01 ng/mL.This method has high sensitivity and can be performed automatically.