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目的探讨转化生长因子-β1(TGF-β1)和内皮素-1(ET-1)在先天性心脏病(先心病)伴肺动脉高压(PH)的肺组织腺泡内肺动脉中的表达及意义。方法收集51例肺组织,其中41例为左向右分流型先心病患儿[伴PH25例(A组),无PH16例(B组)],对照组10例(C组),应用原位杂交、图像分析检测51例腺泡内肺动脉(IAPA)TGF-β1mRNA和ET-1mRNA的表达及平均吸光度值(A值);透射电镜观察肺组织超微结构;弹力纤维(VG)染色显示腺泡内肺动脉并计算其数量的变化。结果(1)肺腺泡内部分肌型及环肌型动脉的数量,A、B两组分别与C组比较,F值分别为149.96、142.01,P<0.01;(2)电镜观察,有的肺小动脉内皮细胞增生;中膜平滑肌增厚,平滑肌细胞面积增大;外膜胶原纤维密集;毛细血管基膜增厚;(3)原位杂交发现,TGF-β1mRNA在A、B两组腺泡内肺动脉均有阳性表达,A值分别为(0.1988±0.0498)、(0.1098±0.0428),C组表达微弱(A值=0.0578±0.0096),A、B、C三组间比较差异有统计学意义(P<0.05);ET-1mRNA在A、B两组肺腺泡内肺动脉内皮细胞表达明显增强,A值分别为(0.1692±0.0205)、(0.1004±0.0140),C组仅有微弱表达(A值=0.0746±0.0119),A、B、C三组间比较差异有统计学意义(P<0.01)。结论左向右分流型先心病患儿肺组织腺泡内部分肌型及肌型动脉数量明显增加,肺血
Objective To investigate the expression and significance of transforming growth factor-β1 (TGF-β1) and endothelin-1 (ET-1) in the pulmonary artery of pulmonary alveoli in patients with congenital heart disease (CHD) and pulmonary hypertension (PH) Methods Totally 51 lung tissues were collected from 41 patients with left-to-right shunt congenital heart disease [with PH25 (group A), without PH16 (group B]] and 10 patients with control group (group C) Hybridization and image analysis were used to detect the expression of TGF-β1 mRNA and ET-1 mRNA and the average absorbance value (A value) in 51 cases of acinar pulmonary artery (IAPA). The ultrastructure of lung tissue was observed by transmission electron microscopy. Within the pulmonary artery and calculate the number of changes. Results (1) The number of muscular and muscular arteries in the alveoli of the lungs was significantly lower than that of the C group (F = 149.96, 142.01, P <0.01). (2) Pulmonary arteriolar endothelial cell hyperplasia; Mesangial smooth muscle thickening, smooth muscle cell area increased; Envelope collagen fibers dense; Thickening of capillary basement membrane; (3) In situ hybridization found that TGF-β1mRNA in A, B two groups of gland The values of A were (0.1988 ± 0.0498) and (0.1098 ± 0.0428) in group B, while the expression in group C was weak (A = 0.0578 ± 0.0096). The differences among group A, B and C were statistically significant (P <0.05). The expression of ET-1 mRNA in pulmonary alveoli of A and B groups was significantly increased (P <0.05), the A values were (0.1692 ± 0.0205) and (0.1004 ± 0.0140) A = 0.0746 ± 0.0119). There was significant difference among A, B and C groups (P <0.01). Conclusion The numbers of muscular and muscular arteries in the alveoli of lung in left-right shunt children with congenital heart disease are significantly increased.