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目的观察丙型肝炎病毒(HCV)持续感染者与自然阴转者外周血HCV准种构成的变化规律。方法应用基因扩增、分子克隆和测序的方法,对未接受过治疗的4例HCV持续感染者与4例自然阴转者前后间隔10年血清中HCV高变区1(HVR1)基因片段进行了序列分析及遗传进化关系比较。结果与持续感染者相比,自然阴转者外周血HCVHVR1区准种群体组内平均遗传距离、熵值较小。4例持续感染者中有3例10年前后血清HCVHVR1准种群体组内与组间遗传距离有明显差异。8例感染者中有7例血清HCV准种KA/KS值大于1。结论在丙型肝炎的自然病程中HCV准种遗传复杂度、变异度大小可能与丙型肝炎的转归相关;HCV准种构成可能发生改变。
Objective To observe the changes of the quasispecies composition of HCV in the peripheral blood of patients with chronic hepatitis C virus (HCV) infection and those who are naturally negative. Methods Four non-HCV survivors were enrolled in this study. The HVR1 gene fragment in serum of 10 unrelated patients with spontaneous remission (n = 6) Sequence Analysis and Genetic Evolutionary Relationships. Results Compared with those with persistent infection, the average genetic distance and entropy in the HCVHVR1 quasispecies group in the naturally negative individuals were smaller. Three of the four persistent infections had significant differences within and between the groups within 10 years of the HCV HCV RV quasispecies population. Seven out of eight infected patients had a serum KA / KS KA / KS value greater than 1. Conclusion The genetic complexity of HCV quasispecies in the natural course of hepatitis C may be related to the prognosis of hepatitis C. The quasispecies constitution of HCV may be changed.