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目的:探讨补阳还五汤对中脑动脉闭塞(MCAO)后局灶性脑缺血大鼠不同时间点脑组织病理形态及一氧化氮(NO)、血管性假血友病因子(v WF)、6-酮-前列腺素F1α(6-keto-PGF1α)表达的影响。方法:用颈内动脉线栓法建立MCAO模型,采用HE染色、TUNEL法观察缺血后1d、3d、7d脑组织病理形态;采用酶联免疫吸附法(ELISA)测定血浆中NO、v WF、6-keto-PGF1α的表达。结果:与模型组同期比较,补阳还五汤组脑组织梗死范围在治疗后7d减少最明显(P<0.01),细胞凋亡率治疗后7d降低最明显(P<0.01);脑缺血后7d,依达拉奉组和补阳还五汤组血浆NO表达水平均较模型组同期明显升高(P<0.01),补阳还五汤组在治疗后7d NO升高最明显(P<0.01);脑缺血后3、7d,依达拉奉组和补阳还五汤组血浆v WF表达水平较模型组同期明显降低(P<0.01),补阳还五汤组在治疗后3d v WF降低最明显(P<0.01),与模型组同期比较,补阳还五汤组治疗后7d6-keto-PGF1α水平显著增加(P<0.01)。结论:补阳还五汤能通过减少脑组织梗死范围、降低细胞凋亡率,下调v WF、上调NO、6-keto-PGF1α水平表达,从而有效保护脑组织。
Objective: To investigate the effect of Buyang Huanwu Decoction on the pathological changes of brain tissue and the levels of nitric oxide (NO), von Willebrand factor (v WF) in focal cerebral ischemia rats after middle cerebral artery occlusion (MCAO) ), 6-keto-prostaglandin F1α (6-keto-PGF1α). Methods: The MCAO model was established by the method of internal carotid artery occlusion. HE staining and TUNEL method were used to observe the pathological changes of brain tissue on the 1st, 3rd, and 7th day after ischemia. The levels of NO, vWF in plasma were detected by enzyme-linked immunosorbent assay (ELISA) 6-keto-PGF1α expression. Results: Compared with the model group, the extent of infarct in Buyang Huanwu Decoction group decreased most obviously on the 7th day after treatment (P <0.01), and the apoptotic rate decreased most obviously on the 7th day after treatment (P <0.01). Cerebral ischemia After 7 days, the plasma levels of NO in Edaravone and Buyang Huanwu Decoction groups were significantly higher than those in the model group (P <0.01), and NO in the Buyang Huanwu Decoction group was the highest at 7 days after treatment (P <0.01). At 3 and 7 days after cerebral ischemia, vWF expression in vasalate group and BYHWD group was significantly lower than that in the model group (P <0.01) 3d v WF decreased most significantly (P <0.01). Compared with the model group, the level of 6-keto-PGF1α increased significantly on the 7th day after treatment in Buyang Huanwu Decoction group (P <0.01). Conclusion: Buyang Huanwu Decoction can effectively protect brain tissue by reducing the range of cerebral infarction, reducing the apoptosis rate, decreasing v WF and up-regulating the expression of NO and 6-keto-PGF1α.