Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic

来源 :Asian Pacific Journal of Tropical Biomedicine | 被引量 : 0次 | 上传用户:jacky899
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Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.Methods: The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis(sodium dodecyl sulfate polyacrylamide gel electrophoresis), western blot, and liquid chromatography–mass spectrometry.Results: The proteins that have molecular weight(MW) 43 and 34 k Da were the antigenic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins(MW: 43 k Da) and two novel hypothetical proteins(MW:34 k Da). Further analysis(BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that two novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi.Conclusions: The identified proteins are potential to be developed as a biomarker of mosquito bite’s exposure. Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools. Methods: The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis (sodium Results: The proteins that have molecular weight (MW) 43 and 34 kDa were the antigenic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins ( Further analysis (BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that both novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi. MW: 43 kDa) and two novel hypothetical proteins Conclusions: The identified proteins are potential to be developed as a biomarker of mosquito bite’s exposure.
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