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【目的】检测到新的控制稻米品质性状相关的QTL并分析各性状间的相关性,为了解控制水稻品质的遗传机理和培育优质水稻品种奠定基础。【方法】利用Sasanishiki×Habataki回交重组自交系(backcross inbred lines,BILs)群体在两个环境下种植的结果,检测与稻米直链淀粉含量、蛋白质含量及RVA谱特征值相关的加性QTL。【结果】表型分析结果显示,Habataki的蛋白含量明显高于Sasanishiki;而除消减值以外其余的稻米品质性状指标,Sasanishiki均高于Habataki。利用BIL群体共检测到加性QTL 42个,其中10个QTL位点在2个环境中均能被检测到,即q PC8、q AC4、q AC10、q PKV2、q PKV7、q HPV7、q CPV1、q BDV4、q BDV7、q SBV7,且q CPV1、q BDV4、q PKV7、q HPV7和q AC10等5个QTL尚未见报道。同时,我们还利用Sasanishiki×Habataki染色体片断置换系(Chromosome segment substitution lines,CSSLs)验证了10个稳定表达的QTL位点。【结论】稻米RVA谱特征值与直链淀粉含量、蛋白质含量之间呈现一定相关性,且控制不同品质性状的QTL之间具有共定位现象。
【Objective】 The purpose of this study was to detect new QTLs related to controlling rice quality traits and to analyze the correlations among the traits in order to lay a foundation for understanding the genetic mechanism controlling rice quality and cultivating high quality rice varieties. 【Method】 The additive QTLs related to rice amylose content, protein content and RVA profile were detected using the results of planting in backcross inbred lines (BILs) of two groups of Sasanishiki × Habataki backcross . 【Result】 The results of phenotypic analysis showed that the protein content of Habataki was significantly higher than that of Sasanishiki. The content of Sasanishiki was higher than that of Habataki except the subtraction value. A total of 42 QTLs were detected in the BIL population, of which 10 QTL loci were detected in 2 environments, namely q PC8, q AC4, q AC10, q PKV2, q PKV7, q HPV7, q CPV1 , Q BDV4, q BDV7, q SBV7, and five QTLs qPCV1, q BDV4, q PKV7, q HPV7 and q AC10 have not been reported yet. At the same time, we also validated ten stable QTLs using Sasanishiki × Habataki Chromosome segment substitution lines (CSSLs). 【Conclusion】 The rice RVA profile has a certain correlation with amylose content and protein content, and co-localization between QTLs controlling different quality traits.