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目的 本实验采用免疫细胞化学方法—Biotin -Streptavidin—Peroxidase(S .P法 ) ,在体外 ,对戊二醛鞣制和混合醇改性后的牛心包进行免疫原性检测。方法 取新鲜牛心包制备抗牛心包抗体 ,采用S .P法在体外进行免疫原性检测。结果 新鲜牛心包表现为强抗原性 ,戊二醛组和混合醇改性组的牛心包 ,抗原性显著降低 ,与对照组明显差异。结论 ⑴戊二醛鞣制的牛心包经混合醇改性处理后 ,免疫原性显著降低。⑵S .P法做为一种体外免疫检测方法 ,具有临床实用价值。
OBJECTIVE: To detect the immunogenicity of bovine pericardium after glutaraldehyde tanning and mixed alcohol modification in vitro using the method of immunocytochemistry-Biotin-Streptavidin-Peroxidase (S.P.). Methods Fresh bovine pericardium was used to prepare anti-bovine pericardial antibody, and the immunogenicity was detected by S-P method in vitro. Results Fresh bovine pericardium showed strong antigenicity. The bovine pericardium and antigenicity of glutaraldehyde group and mixed alcohol-modified group were significantly lower than those of control group. Conclusion ⑴ glutaraldehyde-tannin bovine pericardium mixed alcohol modified, significantly reduced immunogenicity. ⑵ S.P method as an in vitro immunoassay, with clinical practical value.