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作者用半微量测试法研究了15株恶性疟原虫在无药物作用下经2至15个月连续培养期间对氯喹、奎宁和甲氟喹的敏感性。恶性疟原虫患者的血液系用含有ACD抗凝剂的10ml真空瓶采集,在4℃下保存并在72小时内使用。其中6号和14号病人曾每天服用硫酸氯喹100mg,其它13个株的病人抽血前数周未服抗疟药。每个患者的红细胞(RBC)用RPMI培养液洗涤后一部分用作药物敏感试验和贮藏在-196℃,另一部分则与等量的正常红细胞(RBC_5)混合后进行培养。体外培养系按Trager和Jensen法
The authors tested the susceptibility of 15 Plasmodium falciparums to chloroquine, quinine and mefloquine during 2 to 15 months of continuous culture without drug effects using a semi-micro assay. The blood of patients with P. falciparum was harvested using a 10 ml vacuum flask containing ACD anticoagulant, stored at 4 ° C and used within 72 hours. Among them, patients No. 6 and No. 14 took chloroquine sulfate 100 mg daily, and patients in the other 13 strains did not take antimalarial drugs for several weeks before blood drawing. Each patient’s erythrocytes (RBCs) were washed with RPMI broth and then used for drug susceptibility testing and stored at -196 ° C while the other part was mixed with an equal number of RBCs (RBC_5). In vitro culture according to Trager and Jensen method