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近年来,细胞免疫的体外测定方法发展迅速,方法很多,但往往由于方法繁琐,耗血量大,设备要求高,耗费时间长等因素,使其在临床应用中受到一定限制。1972年Halliday和Miller在白细胞移动抑制试验的基础上建立了白细胞粘附抑制试验——LAI试验。其原理是致敏的白细胞在体外与相应的抗原作用后,可使白细胞粘附玻璃表面的能力降低。由于方法简便,迅速、敏感且具一定的特异性,因此很快试用于临床,作为细胞免疫功能测定和肿瘤的诊断,予后判断之用(2～6)。我们用肠癌提取液对20例肠癌患者和40例其他肿瘤患者和健康人进行了LAI试验,以研究其在肠癌诊断上之价值。
In recent years, the in vitro determination of cellular immunity has developed rapidly and has many methods. However, due to complicated methods, high blood consumption, high equipment requirements, and long time consumption, it has been limited in clinical applications. In 1972, Halliday and Miller established the leukocyte adhesion inhibition test LAI test on the basis of the leukocyte migration inhibition test. The principle is that the sensitized leukocytes, after interacting with the corresponding antigens in vitro, can reduce the ability of leukocytes to adhere to the glass surface. Since the method is simple, rapid, sensitive, and specific, it is quickly tested for clinical use as a measure of cellular immune function and diagnosis of tumors for post-judgment (2-6). We used a colon cancer extract to perform LAI tests on 20 patients with colorectal cancer and 40 patients with other cancers and healthy individuals to study its value in the diagnosis of colorectal cancer.