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由于具有放射性非常弱以及对生物组织甚至是细胞无损伤的特点,79Se是对硒元素进行长期无损伤生命科学研究的理想示踪核素7。9Se示踪方法学的建立对于硒的生命科学与生物医学研究将具有十分特殊的意义。加速器质谱测量79Se(79Se-AMS)的生物示踪方法学具有高灵敏度,高准确性以及高精度等特点。通过采用79Se-AMS方法对亚毒性剂量的硒代谢过程进行研究,建立了79Se-AMS生物示踪方法学,重点探索了生物示踪样品的制备流程,化学处理以及样品测量过程。采用分子负离子的引出形式以及四阳极双栅电离室对79Se和79Br两种同量异位素进行鉴别,从而记录实际样品中79Se的原子个数。实验建立了亚毒性剂量硒在Wistar大鼠肾脏内的代谢曲线,为生命科学以及生物医学领域提供了新的测量方法与手段,也为AMS的应用开拓了新的领域。
79Se is an ideal tracer for long-term, non-destructive life science research of selenium due to its very low radioactivity and its non-invasive effects on biological tissues and even its cells. Establishment of 7.9Se Tracer Methodology Selenium’s life sciences and Biomedical research will have very special significance. Accelerator Mass Spectrometry 79Se (79Se-AMS) bio-tracing methodology is characterized by its high sensitivity, high accuracy, and high accuracy. By using 79Se-AMS method to study the selenium metabolism process at sub-toxic dose, a 79Se-AMS bio-tracing method was established, and the preparation flow, chemical treatment and sample measurement of bio-tracer sample were emphatically explored. 79Se and 79Br isobaric isotopes were identified by the extraction of molecular anions and a four-anode dual-gate ionization chamber to record the number of 79Se atoms in the actual sample. The metabolic curves of selenium in Wistar rat kidney were established experimentally, which provided new measurement methods and methods for life sciences and biomedicine, and opened up new fields for the application of AMS.