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目的:筛选体外抑制乙型肝炎表面抗原(HBsAg)有效的中草药,同时探讨筛选方法。方法:采用反向被动血凝抑制试验和自建的中草药提纯抑毒法。结果:从80种中草药中筛出有效药物18种,其中4孔抑制的1种,3孔抑制的3种,2孔抑制的7种,1孔抑制的7种,同时证实了此种筛选方法简捷、快速、敏感、可靠。结论:筛选出的有效药物不仅为临床使用提供了较为可靠的依据,而且自建的筛选方法亦供选用。
OBJECTIVE: To screen effective Chinese herbal medicines for inhibiting hepatitis B surface antigen (HBsAg) in vitro, and to explore screening methods. Methods: Reverse passive hemagglutination inhibition test and self-built Chinese herbal medicine purification and anti-virus method were used. Results: Eighteen kinds of effective drugs were screened out from 80 kinds of Chinese herbal medicines, including 4 inhibitions in 4 wells, 3 inhibitions in 3 wells, 7 inhibitions in 2 wells, and 7 inhibitions in 1 well. At the same time, this screening method was confirmed. Simple, fast, sensitive and reliable. Conclusion: The screening of effective drugs not only provides a more reliable basis for clinical use, but also the selection of self-built screening methods.