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目的建立山东产半夏药材的HPLC指纹图谱,为半夏药材质量控制提供方法和依据。方法采用C18柱(250mm×4.6mm,5μm),以乙腈—水作为流动相进行梯度洗脱,流速1.0mL/m in,检测波长为260nm,对获得的HPLC指纹图谱采用相似度评价软件和SPSS13.0软件进行相似度评价和聚类分析。结果建立了山东产半夏药材的HPLC指纹图谱,方法学考察符合指纹图谱技术要求。相似度计算及聚类分析表明,山东不同产区半夏药材指纹图谱之间相似度高,归为一大类,但与商品半夏药材相似度差异较大、与半夏炮制品、天南星等则不能聚为一类。结论建立的半夏药材高效液相分析方法简单、准确、重现性好,峰分离度较好,可作为半夏药材鉴别和量化控制的依据。
Objective To establish HPLC fingerprinting of Pinellia ternata in Shandong and to provide the method and basis for the quality control of Pinellia ternata. Methods The mobile phase consisted of C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution of acetonitrile-water as the mobile phase. The flow rate was 1.0 mL / min and the detection wavelength was 260 nm. The fingerprint of the obtained HPLC fingerprint was evaluated by using similarity evaluation software and SPSS13 .0 software for similarity evaluation and cluster analysis. Results The HPLC fingerprinting of Pinellia ternata from Shandong Province was established. The methodological study accorded with the technical requirements of fingerprinting. Similarity calculation and cluster analysis showed that the similarity of the fingerprints of the Pinellia ternata from different areas in Shandong was classified as one of the major categories, but the similarities with the products of the Pinellia ternata were quite different. Compared with the products of Pinellia ternata, You can not get together. Conclusion The established HPLC method is simple, accurate, reproducible and has good peak resolution. It can be used as the basis for identification and quantitative control of Pinellia ternata.