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应用Ｄ－半乳糖胺加内毒素脂多糖制成大鼠急性肝损伤模型，并分别于给药后６、１２、２４、４８小时采集肝组织标本，用原子吸收分光光度计（火焰法）定量分析铁钙铜锌锰５种元素含量。结果肝组织中铁、钙随肝损伤的加重逐渐增高，锌则降低；伴随着肝组织中丙二醛含量增高，超氧化物岐化酶活性降低，而铜、锰变化不明显。结果提示：急性肝损伤时，肝组织中有铁超载、钙超载及锌含量的降低。
D-galactosamine plus endotoxin lipopolysaccharide was used to establish acute liver injury model in rats. Liver samples were harvested at 6, 12, 24 and 48 hours after administration, and quantified by atomic absorption spectrophotometer (flame method) Analysis of iron, calcium, copper, zinc, manganese and five elements content. Results The levels of iron and calcium in liver increased gradually with the increase of hepatic injury, while the levels of zinc decreased. With the increase of malondialdehyde in liver tissue, the activity of superoxide dismutase decreased while the changes of copper and manganese did not change obviously. The results suggest that: acute liver injury, liver tissue iron overload, calcium overload and zinc content decreased.