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为建立逆转录病毒介导的多药耐药基因MDR1转移体系，用脂质体转染法将复制缺陷型道病毒载体HaMDR导入鼠源单向型包装细胞GP＋E86；收集单向型逆病毒上清并重复转导双嗜型包装细胞，获得高满度病毒生产细胞PA317／HaMDR。结果：鼠源单向型与双嗜型病毒生产细胞的滴度分别为6．2×105CF/ml和8．5×105CFU／ml，无辅助病毒产生；聚合酶链反应分析证实两种生产细胞均整合有MDR1基因并能持续表达mRNA；流式细胞术与MTT法显示，转移有MDR1基因的包装细胞可高效表达功能性P一糖蛋白，使耐药水平较母株提高12－198倍。该研究为将MDR1基因导入造血于细胞莫定了基础。
To establish a retrovirus-mediated multidrug resistance gene MDR1 transfer system, replication-defective adenovirus vector HaMDR was introduced into murine unilaterally packaged cells GP + E86 by lipofection. The unidirectional retrovirus supernatant The double-type packaging cells were transduced repeatedly to obtain PA317 / HaMDR full-length virus-producing cells. Results: The titers of murine unilateral and biphp virus-producing cells were 6.2 × 10 5 CF / ml and 8.5 × 10 5 CFU / ml, respectively, with no helper virus. Polymerase chain reaction analysis confirmed that the two production cells MDR1 gene was integrated and the mRNA was continuously expressed. Flow cytometry and MTT assay showed that the packaging cells transfected with MDR1 gene could efficiently express functional P-glycoprotein, resulting in a 12-198-fold increase in drug resistance compared with the parent strain. This study laid the foundation for MDR1 gene into hematopoietic cells.