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目的观察临床标本分离菌株对酒精消毒液的敏感性,以了解酒精对致病菌有效杀灭浓度的变化。方法采用细菌定量检测方法,对不同浓度的酒精消毒液作用后的6种细菌存活情况,分析其敏感性变化。结果未经酒精作用的对照组各细菌生长良好,平均生长菌数为1 123 cfu/平板。经体积分数10%、20%、30%、40%和50%酒精作用10min,各细菌平均存活率依次为51%、23%、4%、<1%和0。6种试验菌对酒精敏感性无明显差别。结论临床分离的不同种类细菌对酒精消毒液比较敏感,体积分数50%浓度的酒精可完全杀灭细菌繁殖体。
Objective To observe the sensitivity of isolates of clinical specimens to alcohol disinfectant to understand the effective concentration of alcohol to kill pathogens. Methods Bacterial quantitative detection method was used to study the changes of the six bacteria ’s survival after different concentrations of alcohol disinfectant. Results In the control group without alcohol, the bacteria grew well with an average of 1 123 cfu per plate. The average survival rate of each bacteria was 51%, 23%, 4%, <1% and 0.6 kinds of test bacteria were sensitive to alcohol by the volume fraction of 10%, 20%, 30%, 40% and 50% No significant difference in sex. Conclusions The different kinds of bacteria isolated from clinic are more sensitive to alcohol disinfectant, and alcohol with volume fraction of 50% can completely kill bacterial propagules.