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目的探讨妊娠期孕妇梅毒筛查及确诊的方法学,以提高阳性率;选择母婴阻断是否成功的最佳方法,尽早确诊梅毒的产妇产后婴儿先天梅毒的发生。方法将4863例孕妇采用Trust法,ELISA法进行梅毒检测梅毒孕妇产后婴儿采用PCR法以及TP-Ig M-ELISA检测对照组采用Trust法,ELISA法PCR法进行测定。结果围产期梅毒检测Trust法敏感性为71.2%,特异性为90.0%。ELISA法敏感性为96.2%特异性为98.0%。PCR法敏感性特异性近于100%。先天梅毒确诊试验血液标本Ig M-ELISA法敏感性为66.7%,特异性为98.0%,PCR法敏感性为40.0%,特异性为100.0%.胎盘组织PCR法敏感性特异性近于100%。结论围产期筛查的孕妇选择用ELISA法进行检测筛查,结合PCR确认。先天梅毒出生时的确诊选择胎盘组织处理后进行FQ—PCR技术
Objective To investigate the method of pregnancy screening and diagnosis of syphilis in pregnant women in order to improve the positive rate; the best way to choose the success of maternal and neonatal occlusion, early diagnosis of syphilis maternal congenital syphilis. Methods 4863 pregnant women were tested for the syphilis by ELISA method and ELISA method. The post-natal syphilis pregnant women were detected by PCR method and TP-Ig M-ELISA. The control group was measured by Trust method and ELISA method. Results Perinatal syphilis detection Trust sensitivity was 71.2%, specificity was 90.0%. The sensitivity of ELISA was 96.2% and the specificity was 98.0%. Sensitivity of PCR specificity of nearly 100%. The sensitivity and specificity of IgM-ELISA assay for congenital syphilis were 66.7%, 98.0%, 40.0% and 100.0%, respectively.The specificity of placental PCR was almost 100%. Conclusion Pregnant women during perinatal screening choose ELISA test for screening and confirming with PCR. Confirmatory diagnosis of congenital syphilis at birth placental tissue processing FQ-PCR technology