本文研究了睾丸扭转复位后生精细胞凋亡与iNOS基因表达的关系。采用大鼠建立左侧睾丸扭转复位模型 (72 0 ,2h)。用TUNEL法和免疫组化SP法分别检测扭转复位后第五天生精细胞凋亡和iNOS基因表达。研究发现凋亡主要见于染色质降解的生精细胞 (初级精母细胞和圆形精子细胞 )。间质细胞和支持细胞未见凋亡发生。iNOS表达见于各级生精细胞 ,在染色质降解的生精细胞 (即凋亡细胞 )强表达。本研究表明睾丸扭转复位后生精细胞凋亡增加与iNOS基因表达密切相关。睾丸局部NO生成异常可能是生精细胞凋亡增加的原因之一 This article studies the relationship between spermatogenic apoptosis and iNOS gene expression after testicular torsion and reduction. Rats were used to establish the left testicular torsion reduction model (72 0, 2h). TUNEL and immunohistochemistry SP were used to detect the apoptosis of spermatogenic cells and the expression of iNOS gene on the fifth day after torsional degeneration. The study found that apoptosis is mainly seen in chromatin degradation of spermatogenic cells (primary spermatocytes and round sperm cells). No apoptosis occurred in stromal cells and supporting cells. iNOS expression is found in spermatogenic cells at all levels and is strongly expressed in chromatin-degraded spermatogenic cells (ie, apoptotic cells). This study shows that increased apoptosis of spermatogenic cells after testicular torsion reduction is closely related to iNOS gene expression. One of the reasons for the increased apoptosis of spermatogenic cells may be the abnormal generation of NO in testis