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目的探讨~(60)Coγ射线小剂量辐射损伤对小鼠血液microRNA的影响及意义。方法 SPF级C57BL/6J小鼠接受0.5 Gy全身照射后,分别于照射后6、24 h进行外周血白细胞计数。同时应用Agilent microRNA生物芯片筛选小鼠血液中差异表达microRNA。结果 microRNA芯片筛选出辐射后6 h小鼠中差异表达miRNA共11个,其中7个上调,4个下调。照射24 h小鼠中差异表达miRNA共32个,其中13个上调,19个下调。且与未照射组比较表达差异有统计学意义(P<0.05)。0.5 Gy照射后,6 h和24 h外周血白细胞总数与对照组相比差异无统计学意义(P>0.05)。miRNA表达变化优于血液中白细胞的变化。结论 microRNA在血浆中差异表达与辐射损伤有关,其有望成为辐射损伤新的血液标志物。
Objective To investigate the effect and significance of small dose radiation of ~ (60) Coγ-ray on blood microRNA in mice. Methods Peripheral blood leukocyte counts were performed on SPF grade C57BL / 6J mice at 0.5 Gy whole body irradiation at 6 and 24 h after irradiation. At the same time, Agilent microRNA biochip was used to screen microRNAs differentially expressed in the blood of mice. Results 11 microRNAs differentially expressed in mice 6 h after radiation were screened by microRNA chip, of which 7 were up-regulated and 4 were down-regulated. Thirty-two miRNAs were differentially expressed in 24 h irradiated mice, of which 13 were up-regulated and 19 were down-regulated. The difference between the two groups was statistically significant (P <0.05). There was no significant difference in the total number of WBC at 6 h and 24 h after 0.5 Gy irradiation compared with the control group (P> 0.05). miRNA expression changes over blood leukocyte changes. Conclusion The differential expression of microRNA in plasma is related to radiation damage, which is expected to become a new blood marker for radiation injury.