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建立一种基于Gamma-肽核酸(Gamma-PNA)探针的表面等离子体共振(SPR)基因芯片检测系统,提高检测的灵敏度和特异性。通过自组装分子单层(SAM)技术构建二维结构的表面化学;通过生物信息学方法设计Gamma-PNA,并固定于SAM修饰的SPR芯片表面,优化并确定实验的相关参数。结果表明,Gamma-PNA探针受缓冲液盐离子浓度影响较小,在盐离子浓度为0时仍有良好的杂交反应,并且Gamma-PNA受pH值影响较小,酸性环境更利于杂交。Gamma-PNA探针和传感器技术相结合,既实现了探针的无需标记和实时检测,又提高了杂交的效率和稳定性,为临床应用奠定了基础。
To establish a surface plasmon resonance (SPR) gene chip detection system based on Gamma-peptide nucleic acid (Gamma-PNA) probe to improve the sensitivity and specificity of the detection. Surface chemistry of two-dimensional structure was constructed by self-assembled molecular monolayer (SAM) technique. Gamma-PNA was designed by bioinformatics method and immobilized on the surface of SAM modified SPR chip to optimize and determine the relevant parameters of the experiment. The results showed that the Gamma-PNA probe was less affected by the buffer ion concentration, and had good hybridization reaction when the salt ion concentration was 0, and the Gamma-PNA probe was less affected by the pH value. The acid environment was more conducive to hybridization. The combination of Gamma-PNA probe and sensor technology not only realizes the unmarked and real-time detection of the probe, but also improves the efficiency and stability of hybridization, laying a foundation for clinical application.