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背景与目的:研究食管鳞状细胞癌癌变早期异常改变的蛋白质以发现与食管癌早期病变相关的特征性标志分子。探讨了成束蛋白和膜联蛋白I在食管癌癌前病变中的表达情况。方法:应用免疫组化方法分析食管癌高发现场癌前病变样本中成束蛋白和膜联蛋白I的表达水平,用χ2检验对比成束蛋白和膜联蛋白I在不同程度癌前病变和食管癌中的表达差异。其中包括,食管鳞状细胞癌癌前病变54例、正常食管上皮8例和中晚期食管癌9例成束蛋白的表达水平;以及食管鳞状细胞癌前病变52例、正常食管上皮11例和中晚期食管癌7例的膜联蛋白I表达水平。结果:与正常食管鳞状上皮相比,成束蛋白在食管癌及其癌前病变中表达增强,其表达阳性率为低度癌前病变(轻度和中度不典型增生)85.7%(24/28)、高度癌前病变(重度不典型增生和原位癌)84.6%(22/26)和中晚期食管癌88.9%(8/9)。然而,膜联蛋白I在食管癌及其癌前病变中表达降低或丢失,其表达阳性率分别为低度癌前病变14.3%(4/28)、高度癌前病变8.3%(2/24)和中晚期食管癌0%(0/7)。与正常食管上皮相比,成束蛋白和膜联蛋白I在食管低度癌前病变中表达程度的异常均具有显著意义,P值分别为0.003和0.000。结论:成束蛋白异常增强和膜联蛋白I的丢失与食管癌癌前病变相关。
BACKGROUND & OBJECTIVE: To study the abnormally altered proteins in the early stage of esophageal squamous cell carcinoma (ESCC) in order to find out the characteristic markers associated with the early lesions of esophageal cancer. The expression of fascin and annexin I in precancerous lesions of esophageal cancer was investigated. METHODS: Immunohistochemistry was used to analyze the expression of fasciclin and annexin I in the precancerous lesions of esophageal high-risk area. The chi-square test was used to compare the degree of preneoplastic lesions and esophageal carcinoma with fascin and annexin I. Differences in expression. Including 54 cases of esophageal squamous cell carcinoma precancerous lesions, 8 cases of normal esophageal epithelium and 9 cases of advanced esophageal carcinoma 9 cases of buns; and 52 cases of esophageal squamous cell precancerous lesions, 11 cases of normal esophageal epithelium and The expression of Annexin I in 7 cases of advanced esophageal cancer. RESULTS: Compared with normal esophageal squamous epithelium, the expression of fascin in esophageal carcinoma and its precancerous lesions was enhanced. The positive expression rate was 85.7% in low precancerous lesions (mild and moderately dysplastic). /28), high precancerous lesions (severe dysplasia and carcinoma in situ) 84.6% (22/26) and advanced esophageal cancer 88.9% (8/9). However, annexin I was decreased or lost in esophageal cancer and its precancerous lesions. The positive rates of Annexin I were 14.3% (4/28) for low precancerous lesions and 8.3% (2/24) for high precancerous lesions. And late-stage esophageal cancer 0% (0/7). Compared with normal esophageal epithelium, the abnormal degree of expression of fascin and annexin I in the premalignant precancerous lesions of the esophagus were all significant, with P values of 0.003 and 0.000, respectively. CONCLUSIONS: Abnormal enhancement of fascin and loss of annexin I are associated with precancerous lesions of esophageal cancer.