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【目的】对抗感野生大豆材料根内生细菌的多样性进行比较分析,为研究野生大豆内生细菌与大豆孢囊线虫之间的相互关系奠定基础。【方法】在野生大豆抗大豆孢囊线虫3号生理小种筛选基础上,利用扩增核糖体DNA限制性分析(Amplified ribosomal DNA restriction analysis,ARDRA)和16S rDNA克隆文库测序相结合的方法,对抗感野生大豆根系内生细菌多样性及群落结构进行分析。【结果】野生大豆根内生细菌分属于6大类群,其中变形菌门(Proteobacteria)和厚壁菌门(Firmicutes)为优势类群,相对丰度分别为46.8%和13.6%,另外有少量的放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、酸杆菌门(Acidobacteria)、异常球菌-栖热菌门(Deincoccus-Thermus)和古细菌(Archaea),18.8%克隆序列与环境中未培养细菌的16S rDNA序列有较高的相似性。野生大豆高抗材料内生细菌的多样性比高感材料更为丰富,且抗感材料内生细菌优势菌群存在明显差异,中慢生根瘤菌(Mesorhizobium tamadayense)、肠杆菌(Enterobacter ludwigii)和巨大芽孢杆菌(Bacillus megaterium)为野生大豆高抗材料特有的可操作分类单元(Operational Taxonomic Units,OTUs)中的优势种群。【结论】研究结果表明抗感野生大豆根内生细菌的优势种群存在明显差异,而内生细菌的优势种群与大豆孢囊线虫的相互关系正在深入分析研究。
【Objective】 The objective of this study is to comparatively analyze the diversity of endophytic bacteria in wild-resistant soybean materials and lay the foundation for the study of the relationship between endophytic bacteria and soybean cyst nematode. 【Method】 Based on the screening of wild soybean against soybean cyst nematode No.3, the combination of amplification of the amplified ribosomal DNA restriction analysis (ARDRA) and 16S rDNA clone library sequencing Endophytic Bacteria Diversity and Community Structure of Wild Soybean Roots. 【Result】 The results showed that the endophytic bacteria belonged to 6 groups, of which Proteobacteria and Firmicutes were the dominant groups with the relative abundance of 46.8% and 13.6%, respectively. Actinobacteria, Bacteroidetes, Acidobacteria, Deincoccus-Thermus and Archaea, 18.8% cloned sequences were compared with uncultured bacteria in the environment The 16S rDNA sequence has a high similarity. The diversity of endophytic bacteria in wild soybean highly resistant materials was more abundant than that of high-sensitive materials, and there were significant differences in the dominant bacteria of endophytic bacteria in the resistant soybean plants. Mesorhizobium tamadayense, Enterobacter ludwigii and Bacillus megaterium is the dominant species in Operational Taxonomic Units (OTUs) that is unique to wild soybean highly resistant materials. 【Conclusion】 The results showed that the dominant populations of endophytic bacteria resistant to wild soybean differed significantly. However, the relationship between dominant populations of endophytic bacteria and soybean cyst nematode was deeply analyzed.