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在选择抗某些特殊药物的哺乳动物细胞时,往往伴随有对其它无关药物的交叉抗性,例如一株高度抗秋水仙素的中国仓鼠卵巢细胞(CH~RC5),可同时抗另外8种药物,这被叫做多重抗药型。作者从此种细胞以及抗放线菌素D的叙利亚仓鼠细胞,抗秋水仙素的小鼠细胞,抗长春花碱的人体淋巴样细胞(CEM/VLB100)的多重抗药型中分离出质膜,用十二烷基硫酸钠-聚内酰胺凝胶电泳分析膜的组分,发现其中均存在一种分子量为170,000道尔顿的糖蛋白,即P-糖蛋白,但在药物敏感的亲
Cross-resistance to other unrelated drugs is often associated with the selection of mammalian cells that are resistant to certain special drugs, such as a Chinese colchicine-resistant Chinese hamster ovary cell (CH RC5) that is highly resistant to the other 8 Drugs, this is called multi-drug type. The authors isolated plasma membranes from multiple resistant strains of Syrian hamster cells against actinomycin D, colchicine-resistant mouse cells, and vinblastine-resistant human lymphoid cells (CEM / VLB100) The composition of the membrane was analyzed by sodium dodecyl sulfate-polylactam gel electrophoresis and it was found that a glycoprotein having a molecular weight of 170,000 dalton, P-glycoprotein, was present therein, but in the case of drug-sensitive pro-