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为了证明类固醇反馈调节 LHRH 代谢的机制,法国学者 Drouva SV 进行了下述实验:(1)将去卵巢大鼠分为三组,第一组不加处理作为对照组;第二组植入含17-β雌二醇的硅胶,放置5天;第三组除植入含17-β雌二醇的硅胶外,于第5天再注射孕酮;(2)处死动物后,立即分离下丘脑,经匀浆、离心、提取和滤过等程序,得到两种下丘脑的亚细胞组份,一组为沉淀颗粒,主要含有突触小体;另一组为高
In order to prove that steroid feedback regulates the mechanism of LHRH metabolism, French scholar Drouva SV conducted the following experiments: (1) ovariectomized rats were divided into three groups, the first group without treatment as control group; the second group containing 17 -β estradiol in silica gel for 5 days. The third group was injected with progesterone again on day 5 except for implantation of 17-β-estradiol-containing silica gel. (2) Immediately after the animals were sacrificed, the hypothalamus was separated, After homogenization, centrifugation, extraction and filtration, the subcellular components of the hypothalamus were obtained. One group was precipitated particles and mainly contained synaptosomes. The other group was high