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目的 探讨乙酰肝素酶 (HPSE)mRNA在肝癌组织中的表达及其与肝癌临床病理特征和血管生成的关系。方法 以逆转录 -聚合酶链反应 (RT PCR)检测HPSEmRNA在 5 1例原发性肝细胞癌组织中的表达 ,以Ⅷ因子抗体免疫组织化学染色检测肝癌组织的微血管密度 (MVD)。结果 5 1例肝癌组织中有 2 5例 (4 9.0 % )HPSEmRNA表达阳性。直径≥ 3cm的肝癌组织中 ,HPSE的表达阳性率(6 3.6 % ,2 1/33)明显高于直径 <3cm的肝癌组织 (2 2 .2 % ,4 /18) ,差异有非常显著性 (P <0 .0 1)。高侵袭性肝癌组织中 ,HPSE表达阳性率 (70 .0 % ,14 /2 0 )明显高于中等侵袭性 (4 6 .7% ,7/15 )和低侵袭性肝癌组织 (2 5 .0 % ,4 /16 ;P <0 .0 5 )。微血管密度高的肝癌组织中HPSE表达阳性率 (6 2 .5 % ,2 0 /32 )明显高于微血管密度低的肝癌组织 (2 6 .3% ,5 /19;P <0 .0 5 )。结论 HPSEmRNA表达对肝癌的生长、侵袭和血管生成具有重要作用。
Objective To investigate the expression of heparanase (HPSE) mRNA in hepatocellular carcinoma and its relationship with clinicopathological features and angiogenesis. Methods Reverse transcription-polymerase chain reaction (RT PCR) was used to detect the expression of HPSE mRNA in 51 cases of primary hepatocellular carcinoma. MVD antibody immunohistochemical staining was used to detect the microvessel density (MVD) of hepatocellular carcinoma. Results Of the 51 cases of hepatocellular carcinoma, 25 (49.0 %) HPSE mRNA was positive. The positive rate of HPSE expression in hepatocellular carcinoma with diameter ≥ 3cm (63.6%, 2 1/33) was significantly higher than that of hepatocellular carcinoma with diameter <3cm (2.2%, 4/18), and the difference was very significant ( P <0. 0 1). In highly invasive HCC tissues, the positive rate of HPSE expression (70.0%, 14/20) was significantly higher than that of moderately invasive (46.7%, 7/15) and low-invasive HCC tissues (20.5). %, 4 /16; P <0 .0 5 ). The positive rate of HPSE expression in hepatocellular carcinoma with high microvessel density (62.5%, 20%/32) was significantly higher than that in hepatocellular carcinoma with low microvessel density (26.3%, 5/19; P<0.05). . Conclusion HPSE mRNA expression plays an important role in the growth, invasion and angiogenesis of liver cancer.