论文部分内容阅读
以含油量不同的32份甘蓝型油菜自交纯合系为材料,用直接测序法筛查β-CT亚基的accD编码基因DNA序列的单核苷酸多态性,包括98%的编码区序列共1380 bp。在32份材料中只在材料18和20两个材料中发现了一个相同的SNP,SNP频率为1/1380,单倍型多态性指数Hd=0.121;核苷酸多态性π=0.00009,θ=0.00018。Tajima’s D值为不显著负值,Fu&Li’s检测,各个参数均为不显著正值,Z检验结果中(dN-dS)值也为不显著正值,说明accD基因没有偏离中性进化。系统发育树表明,accD基因在所选的32个油菜材料中是高度保守的,与拟南芥的序列相似度高达95.7%,说明accD基因在物种间也是高度保守的。暗示其在种子油脂合成过程中起着不可替代的作用,此外说明,仅用β-CT亚基编码基因的单核苷酸多态性还不能解释含油量复杂的表现型,还需在更大的群体资源中扩增accD编码基因和其它亚基编码基因进行单核苷酸多态性与含油量表型的相关性研究。
The nucleotide sequences of the accD coding genes of β-CT subunit were screened by direct sequencing using self-cross homozygous lines of 32 different oil-content Brassica napus cultivars. The single nucleotide polymorphisms of 98% coding region The sequence is 1380 bp in length. In 32 materials, only one SNP was found in two materials 18 and 20, the frequency of SNP was 1/1380, the haplotype polymorphism index Hd was 0.121, the nucleotide polymorphism π was 0.00009, θ = 0.00018. Tajima’s D value was not significantly negative, Fu & Li’s test, all parameters were insignificant positive value, Z test results (dN-dS) value is not significant positive, indicating accD gene did not deviate from the neutral evolution. The phylogenetic tree indicated that the accD gene was highly conserved among the 32 selected rapeseed materials, and its sequence similarity with Arabidopsis thaliana was as high as 95.7%, indicating that the accD gene is also highly conserved among species. Suggesting that it plays an irreplaceable role in the synthesis of seed oil. In addition, single nucleotide polymorphism of β-CT subunit coding gene can not explain the complex oil content phenotype, Of population resources amplification accD coding genes and other subunit-encoding genes single nucleotide polymorphism and oil content phenotype correlation study.