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AIM:To observe the regulatory effect of Chinese herbalmedicine on peritoneal lymphatic stomata and itssignificance in treating ascites in liver fibrosis model mice.METHODS:Two Chinese herbal composite prescriptionsware used separately to treat the carbon tetrachloride-induced mouse model of liver fibrosis.The histo-pathologicchanges of the liver sections(HE and VG stainings)wereobserved.The peritoneal lymphatic stomata was detected byscanning electron microscopy and computer imageprocessing.The changes of urinary volume and sodium ionconcentration were measured.RESULTS:In the model group,lots of fibrous tissue formedin liver and extended into the hepatic Iobules to separatethem incompletely.In the treated and prevention groups,the histo-pathologic changes of liver was rather milder,,onlyshowed much less fibrous tissue proliferation in the hepaticIobulea.The peritoneal lymphatic stomata enlarged withincreased density in the experimental groups(diameter:PA,3.07±0.69μm;PB,2.82±0.37μm;TA,3.25±0.82μmand TB,2.82±0.56μm;density:PA,7.11±1.90 stomata·1000 μm~(-2);PB,8.76±1.45 stomata.1000μm~(-2);TA,6.55±1.44 stomata·1000μm~(-2)and TB,8.76±1.79 stomata·1000μm~(-2)),as compared with the model group(diameter:2.00±0.52 μm;density:4.45±1.05 stomata·1000μm~(-2)).Aftertreatment,the urinary volume and sodium ion excretionincreased in the experimental groups(PA,231.28±41.09mmol·L~(-1);PB,171.69±27.48 mmol·L~(-1)and TA,231.44±34.12 mmol·L~(-1)),which ware significantly different with thosein the model group(129.33±36.75 mmol·L~(-1)).CONCLUSION: Chinese herbal medicine has marked effects in alleviating liver fibrosis, regulating peritoneal lymphatic stomata. improving the drainage of ascites from peritoneal cavity and causing increase of urinary volume and sodium ion excretion to reduce the water and sodium retention, and thus have favorable therapeutic effect in treating ascites.
AIM: To observe the regulatory effect of Chinese herbalmedicine on peritoneal lymphatic stomata and itssignificance in treating ascites in liver fibrosis model mice.METHODS:Two Chinese herbal composite prescriptions used used to treat the carbon tetrachloride-induced mouse model of liver fibrosis.The histo- Pathologicchanges of the liver sections(HE and VG stainings)wereobserved.The peritoneal lymphatic stomata was detected byscanning electron microscopy and computer image processing.The changes of urinary volume and sodium ionconcentration were measured.RESULTS:In the model group,lots of fibrous tissue formedin liver And extended into the hepatic Iobules to separatethem incompletely.In the treated and prevention groups,the histo-pathologic changes of liver was rather milder,,onlyshowed much less fibrous tissue proliferation in the hepaticIobulea.The peritoneal lymphatic stomata enlarged withincreased density in the experimental groups (diameter: PA, 3.07±0.69 μm; PB, 2.82±0.37 μm; TA, 3.25±0.82 μmand TB, 2.82±0.56 μm; density: PA, 7.11±1.90 stomata·1000 μm~(-2); PB, 8.76±1.45 stomata.1000 μm~(-2); TA, 6.55±1.44 stomata 1000μm~(-2)and TB, 8.76±1.79 stomata·1000μm~(-2)), as compared with the model group (diameter: 2.00±0.52 μm; density: 4.45±1.05 stomata·1000μm~(-2)) ).Aftertreatment, the urinary volume and sodium ion excretion increased in the experimental groups (PA, 231.28±41.09 mmol·L~(-1); PB, 171.69±27.48 mmol·L~(-1) and TA, 231.44±34.12 mmol ·L~(-1)),which ware significantly different with those in the model group(129.33±36.75 mmol·L~(-1)).CONCLUSION: Chinese herbal medicine has marked effects in alleviating liver fibrosis, regulating peritoneal lymphatic stomata. Improving the drainage of ascites from peritoneal cavity and confused increase of urinary volume and sodium ion excretion to reduce the water and sodium retention, and thus have good therapeutic effect in treating ascites.