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目的:建立高效液相色谱法(HPLC)测定哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量及临床疗效观察。方法:采用高效液相法测定含量,色谱柱为Agilent TC-C18(4.6 mm×250 mm,5μm),以甲醇:0.01 mo L/L硫酸铵溶液(37:63,用三乙胺调p H至4.0)为流动相,流速为1 m L/min,柱温:25℃,检测波长在320 nm。用完全随机法分实验组和对照组进行比较哈医祛风膏疗效。结果:去氢骆驼蓬碱的线性范围为12~83μg(r=0.999 8,n=6)平均回收率为100.14%,RSD值为1.33%、骆驼蓬碱的线性范围为12~83μg(r=0.999 8,n=6)平均回收率为98.89%,RSD值为1.77%。疗效比较治疗组的总有效率为97.3%、对照组的总有效率为90.1%,2组有显著性差异,P<0.01。表明治疗组总体疗效明显优于对照组。结论:高效液相色谱法方法简便,准确,可靠,可以用于哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量测定。并哈医祛风膏治疗颈肩腰腿痛的效果较好、疗效确切、安全性良好,可降低患者复发率,可考虑在临床上推广。
OBJECTIVE: To establish a HPLC method for the determination of the content of harmine and harmalaide in harbamine expelling cream and its clinical efficacy. Methods: The content was determined by high performance liquid chromatography with Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm), methanol: 0.01 mol L / L ammonium sulfate solution (37:63, To 4.0) as the mobile phase at a flow rate of 1 m L / min. The column temperature was 25 ° C and the detection wavelength was at 320 nm. Completely randomized experimental group and control group to compare the effect of Ha medical expelling wind cream. Results: The average recoveries of desalin in the range of 12-83 μg (r = 0.999 8, n = 6) were 100.14% with RSD of 1.33%, and the linear range of harmalal alkali was 12-83 μg (r = 0.999 8, n = 6) The average recovery was 98.89% and the RSD was 1.77%. The total effective rate of the treatment group was 97.3%, while that of the control group was 90.1%. There was a significant difference between the two groups (P <0.01). Show that the overall efficacy of treatment group was significantly better than the control group. Conclusion: The HPLC method is simple, accurate and reliable and can be used to determine the content of harmala and alkali in Harp ’s Qufeng Decoction. And Ha medicine expelling wind cream treatment of neck and shoulder low back pain better, curative effect is safe, good, can reduce the recurrence rate of patients, can be considered in the clinical promotion.