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目的:本研究从形态学角度,观察补充活性肽对大鼠运动后骨骼肌微细损伤的作用。方法:雄性SD大鼠150只,按照补充不同物质(水、大豆分离蛋白和活性肽),运动与不运动,以及运动后0 hr、12 hr2、4 hr和48hr不同时间随机分为15组,所有动物平衡膳食1周后进行实验。运动组大鼠进行持续跑台训练120分钟。实验组和对照组所有动物在平衡膳食期间每天分别以15%活性肽饮料2毫升、15%大豆分离蛋白2毫升和等量的纯净水灌胃。结果:补充活性肽和大豆分离蛋白均可减轻大鼠1次离心运动后骨骼肌微细损伤的形态学改变和超微结构变化,其中活性肽的作用有强于大豆分离蛋白的趋势。
OBJECTIVE: In this study, we observed the effect of peptide supplementation on the microscopic injury of skeletal muscle after exercise in rats. Methods: 150 male Sprague-Dawley rats were randomly divided into 15 groups at different times of 0 hr, 12 hr2, 4 hr and 48 hr after supplemented with different substances (water, soybean protein isolate and active peptide), exercise and no exercise, All animals were balanced for a week after the experiment. Rats in exercise group continued treadmill training for 120 minutes. All animals in experimental group and control group were orally administered with 2 ml of 15% active peptide beverage, 2 ml of 15% soy protein isolate and the same amount of pure water every day during the balanced diet. RESULTS: Supplementation of active peptides and soy protein isolates could reduce the morphological changes and ultrastructural changes of skeletal muscle microdama after one-time eccentric exercise in rats. The activity of active peptides was stronger than that of soy protein isolate.