论文部分内容阅读
目的探讨IL-15是否能够有效纠正脓毒症小鼠的免疫抑制,改善脓毒症小鼠的生存率。方法将铜绿假单胞杆菌经尾静脉注射至C57雄性小鼠构建脓毒症小鼠模型,模型小鼠分为3组:对照组和0.5、1μg IL-15组,分别腹腔注射生理盐水,0.5、1μg IL-15。观察脓毒症小鼠的生存时间,计算脏器细菌定植数量,检测小鼠T细胞和NK细胞的含量,并测定小鼠外周血细胞因子IFN-γ的表达水平。结果 0.5、1μg IL-15组小鼠生存时间为(3.8±3.9)和(5.0±4.9)d,明显长于对照组[(1.9±0.9)d]。腹腔注射IL-15后,小鼠脾脏细菌的定植量[0.5μg组:(15.4±2.9)×103CFU/μg,1μg组:(9.7±1.5)×103CFU/μg]显著低于对照组[(29.8±3.2)×103CFU/μg,P<0.01]。腹腔注射1μg IL-15有效的增加了小鼠T细胞[(69.84±13.28)%vs(58.63±6.25)%,P<0.01]、NK细胞的数量[(20.96±5.78)%vs(9.83±1.36)%,P<0.01]和IFN-γ的表达水平[(526.1±23.8)pg/ml vs(289.4±16.2)pg/ml,P<0.01]。结论 IL-15能够有效的纠正脓毒症小鼠的免疫抑制,充分活化天然免疫系统和获得性免疫系统,从而改善脓毒症小鼠的生存状况。
Objective To investigate whether IL-15 can effectively correct the immunosuppression in septic mice and improve the survival rate in septic mice. Methods Pseudomonas aeruginosa was inoculated into C57 male mice via caudal vein to establish a mouse model of sepsis. The model mice were divided into three groups: control group and 0.5 and 1 μg IL-15 groups, respectively, with saline, 0.5 , 1 μg IL-15. The survival time of the septic mice was observed. The number of organ colonies was counted. The T cells and NK cells in mice were detected. The expression of IFN-γ in peripheral blood was measured. Results The survival time of 0.5,1μg IL-15 group was (3.8 ± 3.9) and (5.0 ± 4.9) days, significantly longer than that of the control group [(1.9 ± 0.9) days]. Intraperitoneal injection of IL-15 resulted in a significantly lower colonization rate of spleen bacteria in mice (0.5μg group: (15.4 ± 2.9) × 103CFU / μg, 1μg group: (9.7 ± 1.5) × 103CFU / μg] ± 3.2) × 103 CFU / μg, P <0.01]. The intraperitoneal injection of 1μg IL-15 effectively increased the number of T cells in mice [(69.84 ± 13.28)% vs (58.63 ± 6.25)%, P <0.01] and the number of NK cells [(20.96 ± 5.78)% vs )%, P <0.01] and the level of IFN-γ [(526.1 ± 23.8) pg / ml vs (289.4 ± 16.2) pg / ml, P <0.01]. Conclusion IL-15 can effectively correct immunosuppression in septic mice and fully activate the natural immune system and the adaptive immune system to improve the survival of septic mice.