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本文介绍了玻璃微电极传统的充灌方法改进。我们称之为半自动充灌法。这种充灌法不仅具备自动充灌法简便而快速的优点,而且因毛坯细玻管中不放纤维细管,与自动充灌法相比,拉制成尖径小于1μm 的细胞内微电极比较容易。采用这种充灌方法的关键是毛坯细玻管的清洁处理时,与常规方法不同。
This article describes the improvement of traditional filling methods for glass microelectrodes. We call it semi-automatic filling method. This filling method not only has the advantages of simple and fast automatic filling method, but also does not put fine tube in the rough tube thin tube, compared with the automatic filling method, pulled into the tip diameter less than 1μm intracellular microelectrode easy. The key to using this method of filling is different from the conventional method for the cleaning of rough glass tubes.