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由光密度对酪氨酸酶浓度作图 ,判断乙醇对酪氨酸酶催化氧化左旋多巴反应的抑制作用是可逆的 ;乙醇浓度变化时用双倒数法判定乙醇与单独的酪氨酸酶不发生作用 ,只有当酪氨酸酶与底物结合时乙醇才以之作用。
By the optical density of tyrosinase concentration mapping to determine the inhibition of ethanol on tyrosinase catalytic oxidation of levodopa reaction is reversible; ethanol concentration changes using double reciprocal determination of ethanol and tyrosinase alone In effect, ethanol acts only when tyrosinase binds to the substrate.