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目的探讨绿茶多酚对高脂膳食诱导代谢综合征大鼠血管通透性升高的干预效果。方法雄性wist-ar大鼠随机分为正常对照、高脂对照、茶多酚低、中、高剂量干预组,断乳后开始给予高脂饮食,达到成年体重后进行茶多酚干预,持续30周,测定大鼠体重、脂肪系数、血糖、血脂水平,采用伊文思蓝注射法测定大鼠血管通透性,硝酸根还原酶法测定大鼠血清一氧化氮(NO)水平,采用试剂盒测定内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)活性,制作血管冷冻切片,采用二氢乙啶荧光探针检测其活性氧分子(ROS)水平。结果与对照组(0.16±0.007)μg/mg比较,高脂组大鼠血管通透性(0.19±0.006)μg/mg明显升高,与高脂组比较,低、中、高茶多酚组大鼠血管通透性[(0.16±0.004)、(0.14±0.010)、(0.15±0.010)μg/mg]明显降低;茶多酚组ROS水平明显低于高脂组;与高脂组比较,茶多酚明显上调eNOS活性,而下调iNOS活性。结论绿茶多酚对代谢综合征大鼠血管通透性升高具有保护作用,其保护效应可能与减轻氧化损伤有关。
Objective To investigate the intervention effect of green tea polyphenols on vascular hyperpermeability in rats with metabolic syndrome induced by high fat diet. Methods Male wist-ar rats were randomly divided into normal control group, high-fat control group, low, medium and high dose of tea polyphenols intervention group. After weaning, high-fat diet was started. After reaching adult body weight, The body weight, fat coefficient, blood glucose and blood lipid level of rats were measured. The vascular permeability of rats was measured by Evans blue injection method. The level of serum nitric oxide (NO) was determined by nitrate reductase method. Endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) were used to prepare frozen sections of blood vessels. The level of reactive oxygen species (ROS) was detected by dihydrogen bromide fluorescent probe. Results Compared with the control group (0.16 ± 0.007) μg / mg, the vascular permeability (0.19 ± 0.006) μg / mg in the hyperlipidemia group was significantly increased. Compared with the hyperlipidemia group, the low, middle and high tea polyphenols The vascular permeability in rats was significantly lower than that in high-fat group [(0.16 ± 0.004), (0.14 ± 0.010) and (0.15 ± 0.010) μg / mg], while the ROS level in tea polyphenols was significantly lower than that in high- Tea polyphenols significantly upregulated eNOS activity but down-regulated iNOS activity. Conclusion Green tea polyphenols have a protective effect on vascular permeability of rats with metabolic syndrome, and its protective effect may be related to the reduction of oxidative damage.