The aim of this work was to develop a mutation and selection procedure to select the high-yield strain,and this strain could alleviate the inhibition in the biosynthesis of ramoplanin caused by L-ieucine. In this experiment, the strain Actinoplanes sp.C6-78-14 that produced 384.8 mg of total productivity per liter was isolated.Its ramoplanin productivity was 4.13-fold higher thanthat of the parent strain A ctinoplanes sp.C2-9-45.The subculture experiments showed that the hereditary character of C6-78-14 was stable.Subsequent media studies with swain Actinoplanes sp.C6-78-14 showed that adding 0.001% FeSO4·7H2O, 0.001% COCl2·6H2O and 0.001% CUSO4·5H2O to fermentation medium respectively could enhance the ramoplanin production, and the production of each group was increased by 10%,15% and 20% compared with the control group.