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利用滴涂于玻碳电极(GCE)表面的以Nafion膜中负电性的磺酸基与天青I(AI)阳离子之间的静电作用,实现天青I在电极上的固定化,再通过静电吸附和自组装技术将纳米CdS吸附的辣根过氧化物酶(HRP)修饰到电极表面,制备出性能良好的H2O2生物传感器。采用循环伏安法(CV)和计时电流法考察了该传感器的电化学性质。实验表明,该传感器具有良好的生物催化活性,较高的灵敏度,良好的选择性和稳定性,且易于制作等特点。响应电流与H2O2浓度在8.0×10-6~1.6×10-3mol/L范围呈现良好的线性关系,检出限为2.72×10-6mol/L。
The immobilization of azure I on the electrode was achieved by the electrostatic interaction between the negatively charged sulfonic acid group and the azure I (AI) cation in the Nafion membrane, which was applied onto the surface of glassy carbon electrode (GCE) Adsorption and self-assembly technology to nano-CdS adsorption of horseradish peroxidase (HRP) modified to the electrode surface to prepare a good performance of H2O2 biosensor. The electrochemical properties of the sensor were investigated by cyclic voltammetry (CV) and chronoamperometry. Experiments show that the sensor has good biocatalytic activity, high sensitivity, good selectivity and stability, and is easy to make and so on. The response current showed a good linear relationship with the concentration of H2O2 in the range of 8.0 × 10-6 ~ 1.6 × 10-3mol / L with the detection limit of 2.72 × 10-6mol / L.