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本实验采用间接免疫荧光技术,观察了小鼠腹腔巨噬细胞和肠系膜淋巴结细胞胞浆微管经~(60)Coγ射线照射后的变化及分布特点;同时还对此变化进行了动态观察、研究;并在透射及扫描电镜下观察了~(60)Coγ射线对胞浆内微管、中心粒和表面微绒毛的影响。实验结果表明:(1)辐照对巨噬、淋巴细胞内胞浆微管的分布及荧光染色模式有明显影响,并且与辐照剂量有关;(2)经一定时间后,由辐照所致的胞浆微管变化可以完全恢复;(3)透射电镜下可见辐照后胞浆微管、中心粒基本消逝,扫描电镜下可见经辐照后细胞表面突起或微绒毛发生明显变化。
In this experiment, indirect immunofluorescence techniques were used to observe the changes and distribution characteristics of cytosolic microtubules of mouse peritoneal macrophages and mesenteric lymph node cells irradiated by 60 Co γ-rays, and to observe and study the changes. The effects of ~(60)Co γ-rays on intracytoplasmic microtubules, centriole and surface microvilli were observed under transmission and scanning electron microscopy. The experimental results show that: (1) Irradiation has a significant effect on the distribution of macrophages, intracellular cytoplasmic microtubules and fluorescent staining patterns, and is related to the dose of radiation; (2) caused by irradiation after a certain period of time. The changes of cytoplasmic microtubules can be completely restored; (3) The cytoplasmic microtubules and centriole can basically disappear after irradiation under TEM. Under irradiation electron microscope, the cell surface protrusion or microvilli can be significantly changed after irradiation.