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在pH 7.40,20.00±0.05℃,磷酸盐缓冲液中,研究了Cu(Ⅱ)及Cu(Ⅱ)络合物的可见光谱,以及DNA存在下,Cu(Ⅱ)的去甲斑蝥酸络合物Cu(Ⅱ)/H_2DCA催化抗坏血酸(H_2A)有氧氧化动力学,检测了Cu(Ⅱ)/H_2DCA催化H_2A有氧氧化过程中·OH生成的速度。实验结果表明,在Cu(Ⅱ)/H_2DCA存在下,抗坏血酸断裂DNA链的反应体系中,存在Cu(Ⅱ)与H_2DCA和DNA的三元络合。据此,推测H_2A的催化有氧氧化对DNA链的断裂作用按特定部位的Fenton反应机理进行。该机理能够解释前文的DNA链断裂实验的所有结果。
The visible spectra of Cu (Ⅱ) and Cu (Ⅱ) complexes were investigated in phosphate buffer at pH 7.40 and 20.00 ± 0.05 ℃, and the presence of DNA in the presence of deoxycantharidate complex of Cu (Ⅱ) The kinetics of aerobic oxidation of ascorbic acid (H_2A) was catalyzed by Cu (Ⅱ) / H_2DCA and the formation rate of · OH during the aerobic oxidation of H_2A catalyzed by Cu (Ⅱ) / H_2DCA was examined. The experimental results show that in the presence of Cu (Ⅱ) / H_2DCA, the ternary complex of Cu (Ⅱ) with H_2DCA and DNA exists in the reaction system of ascorbic acid cleaving DNA. Therefore, we speculated that H 2 A catalytic oxidation of DNA damage on the DNA strand by a specific site of the Fenton reaction mechanism. This mechanism can explain all the results of the previous DNA strand breakage experiment.