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关于人骨髓细胞体外培养的方法已有较多的报导。骨髓细胞体外琼脂培养时形成细胞团(CFU-C)需要细胞团刺激因子(CSF)的参与。过去曾用猴或人的白细胞作为营养层获得良好的结果。也曾用人的白细胞(特别是单核细胞)、胚胎肾或胎盘等制成条件培养液都显示出一定的活
There have been many reports on the method of in vitro culture of human bone marrow cells. The formation of cell clusters (CFU-C) when bone marrow cells are cultured in vitro requires the involvement of cell-derived stimulators (CSFs). In the past, monkey or human leukocytes were used as a nutrient layer to get good results. Also used human white blood cells (especially mononuclear cells), embryonic kidney or placenta made of conditioned medium showed a certain activity