本文报道了全盘自动化高效液相色谱仪基本软件的设计原理和方法。发展的软件实现了色谱仪的控制和反馈的所有功能。实验表明,该软件不仅能可靠地完成色谱仪硬件的控制,光二极管列阵快速扫描检测器的数据采集和处理,而且具备仪器自我诊断能力、色谱方法的设计、色谱的定性和定量等功能。 This paper reports the design principles and methods of the basic software of a total automated HPLC. The developed software implements all the features of chromatographic control and feedback. Experiments show that the software can not only complete the control of chromatographic hardware, but also acquire and process the data of the rapid scan detector of the optical diode array. Moreover, it has the functions of instrument self-diagnosis, chromatographic method design, chromatography qualitative and quantitative.