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目的 研究细脚拟青霉多糖 的分离纯化方法、相对分子量、单糖组成及其结合方式。方法 室温下水提取出粗多糖 ,采用 Sephadex G- 10 0柱层析纯化。经过酸全水解 ,利用阴离子交换柱测定单糖的组成。甲基化分析测定单糖的结合方式。IR以及 NMR光谱的研究确定糖苷键的类型。结果 经高效液相色谱检测为均一性组成 ,从以后的 GC- MS及 NMR图谱也获得证明。结论 细脚拟青霉多糖 为α- (1→ 6 )连结的葡聚糖 ,相对分子量为 2 .0 5×10 4u。
Objective To study the isolation, purification, relative molecular weight, monosaccharide composition and the combination of the polysaccharides of Paecilomyces pinophila. Methods The crude polysaccharide was extracted at room temperature and purified by Sephadex G-100 column chromatography. After acid hydrolysis, the composition of monosaccharides was determined using an anion exchange column. Methylation analysis determines the binding of monosaccharides. Studies of IR and NMR spectra determine the type of glycosidic bond. RESULTS: High-performance liquid chromatography was used to determine the homogeneity of the composition, which was also confirmed by the subsequent GC-MS and NMR spectra. Conclusion Paecilomyces pinophilus is α-(1→ 6)-linked dextran with a relative molecular mass of 2.05×10 4u.