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玉米花药诱导培养,PNC培养基与N_6相比,其诱导频率提高3—25倍,并且使胚状体频率得到大幅度增加;玉米雄穗在低温0—8℃条件下保存13—21天,花药接种在PNC培养基上,能不同程度提高花粉出愈频率,尤以低温处理十九天的诱导效果最好。玉米花粉苗的移栽,是玉米花培育种约重要一环,经过几年的试验研究,采用“浸水练苗移栽技术”可以使花粉苗移栽成活率提高到70％以上。
Compared with N_6, the induction frequency of PNC medium increased by 3-25 folds, and the frequency of embryoid body was greatly increased. Corn tassel was stored for 13-21 days at 0-8 ℃, Anther inoculated on PNC medium, to varying degrees, improve the frequency of pollen, especially the low temperature treatment nineteen days the best induction. The transplanting of corn pollen seedlings is an important part of popcorn breeding. After several years of experimental research, the transplanting survival rate of pollen seedlings can be increased to more than 70% by using “immersion seedling transplanting technique”.