人类隐孢子虫病在近两年才有报道。作者于1984年对南卡罗来纳医院病人的大便标本进行检查,对发现有隐孢子虫者,采集其家庭成员的粪便,同其他健康人的标本一起按双盲法编号,在采集粪便后3小时内进行处理,对隐孢子虫阳性的所有腹泻大便作肠致病菌培养。检查寄生虫采用直接生理盐水涂片,乙烯福尔马林醋酸盐沉淀、碘液固定及改良抗酸染色法。 Cryptosporidiosis has not been reported in the recent two years. The author examined the stool specimens of patients from a South Carolina hospital in 1984 and collected feces of members of his family from those who were found to have cryptosporidium. The specimens were stamped double-blinded together with specimens of other healthy individuals within 3 hours after collecting the stool For processing, all diarrhea stool positive for Cryptosporidium for pathogenic bacteria culture. Parasites were examined by direct saline smear, ethylene formalin acetate precipitation, iodine fixation and modified acid-fast staining.