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目的:探讨雷帕霉素对前列腺癌细胞22RV1增殖及对S6 Kinase 1(S6K1)活性的影响。方法:用不同浓度(0、50、100、200、400 nmol/L)雷帕霉素作用于体外培养的前列腺癌细胞22RV1,MTT法检测细胞生长抑制率;应用液闪激酶活性测定法检测不同浓度雷帕霉素对S6K1活性的影响。结果:雷帕霉素能显著抑制22RV1细胞的生长,呈现明显的量-效关系,随着雷帕霉素剂量的增加,细胞生长抑制率逐渐升高,400 nmol/L的雷帕霉素对22RV1细胞的抑制率最高(P<0.01);同时雷帕霉素还能使S6K1蛋白活性下降,随剂量增高降低越明显,400 nmol/L的雷帕霉素使S6K1蛋白活性下降最明显(P<0.01)。结论:雷帕霉素可通过调控mammal Target ofrapamycin(mTOR)下游蛋白S6K1表达来有效地抑制前列腺癌细胞22RV1的增殖。
Objective: To investigate the effect of rapamycin on the proliferation of prostate cancer cell line 22RV1 and the activity of S6 Kinase 1 (S6K1). Methods: Different concentrations of rapamycin (0, 50, 100, 200 and 400 nmol / L) were used to effect the proliferation of human prostate cancer cell line 22RV1. MTT assay was used to detect the cell growth inhibition rate. Effect of rapamycin on S6K1 activity. Results: Rapamycin could significantly inhibit the growth of 22RV1 cells, showing a significant dose-response relationship. With the increase of rapamycin dosage, the cell growth inhibition rate gradually increased. The ratio of rapamycin 400 nmol / L (P <0.01). At the same time, rapamycin also decreased the activity of S6K1 protein and the more obvious the decrease of S6K1 protein with the increase of dose, the most significant decrease of S6K1 protein activity was observed with rapamycin 400 nmol / L (P <0.01). Conclusion: Rapamycin can effectively inhibit the proliferation of prostate cancer cell line 22RV1 by regulating the expression of mammalian target ofrapamycin (mTOR) downstream protein S6K1.