论文部分内容阅读
利用噬菌体展示技术已选出了多条与靶结合的肽.然而,即使是体内直接筛选得到的,肽与肿瘤或靶器官的体内结合并不理想.为了更好地理解噬菌体在体内的循环,通过MAG399mTc标记噬菌体肽库,研究了肽库在体内分布.体内分布实验结果显示,99mTc-噬菌体主要分布在肝和脾中,而心脏、肌肉、脑和胰腺这些器官或组织中的分布非常低.99mTc-噬菌体在胃、肠和骨中的累积,随着时间延长在不断升高,其他器官中的吸收则在不断降低.从5min到30min,99mTc-噬菌体在血中清除迅速.当噬菌体在体内循环足够长的时间后,一些噬菌体颗粒可以穿透血管进入并内化在器官或组织中.总之,为了筛选具有高特异性和亲和性的肽,应该根据靶器官和筛选部位的不同,在筛选前确定合适的噬菌体在体内的循环时间.
Several phage display peptides have been selected for phage display, however, the in vivo binding of the peptide to the tumor or target organ is not ideal even in direct in vivo screening.In order to better understand the phage in vivo circulation, The phage library of MAG399mTc labeled phage peptide library was used to study the distribution of peptide library in vivo.The results of in vivo distribution showed that 99mTc-phage were mainly distributed in the liver and spleen, whereas the distribution in heart, muscle, brain and pancreas was very low. The accumulation of 99mTc-phage in the stomach, intestine and bone increased continuously with time, while the absorption in other organs kept decreasing. From 5min to 30min, 99mTc-phage cleared rapidly in the blood.When phage were in vivo After cycling for a sufficient period of time, some of the phage particles can penetrate into the blood vessels and internalize into organs or tissues.In summary, in order to screen for peptides with high specificity and affinity, screening should be based on differences in target organs and screening sites Before determining the appropriate phage circulating time in the body.