来源于人类诱导多能干细胞(人iPS细胞)的肝细胞是药物和细胞治疗空前的资源。但是,在体外由人类诱导多能干细胞定向分化成成熟的肝细胞仍然是具有挑战性的。迄今的研究很少注意到那些用于肝细胞分化的hiPSC细胞系间的差异。在本研究中,我们发展了一种改良的肝细胞分化的流程,比较了不同体细胞来源及应用不同载体如逆转录病毒、仙台病毒或附着体质粒所 Hepatocytes derived from human induced pluripotent stem cells (human iPS cells) are an unprecedented resource for drug and cell therapy. However, directed differentiation of human pluripotent stem cells into mature hepatocytes in vitro remains challenging. The studies to date seldom note the differences among the hiPSC cell lines used for hepatocyte differentiation. In this study, we developed an improved protocol for hepatocyte differentiation that compares different sources of somatic cells and the use of different vectors such as retroviruses, Sendai virus or attached plasmids