针对进口大豆的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)检测,建立了一步法RT-PCR和一步法实时荧光RT-PCR检测方法。依据BPMV的外壳蛋白编码基因设计了特异引物和特异Taqman探针,特异引物的扩增片段约为500bp,阳性质粒的实时荧光PCR方法的检测下限为20fg/μL,是一步法RT-PCR方法的100倍,检测时间由约8 h缩短至4 h。种脐灰色斑驳的大豆种子BPMV检测呈阳性,种脐黑色斑驳的大豆种子BPMV检测呈阴性。 In order to detect Bean pod mottle virus (BPMV) imported soybean, a one-step RT-PCR and one-step real-time fluorescence RT-PCR method were established. Based on the coat protein coding gene of BPMV, specific primer and specific Taqman probe were designed. The amplification range of specific primers was about 500 bp. The detection limit of real-time PCR for positive plasmids was 20 fg / μL. 100 times, detection time shortened from about 8 h to 4 h. BPMV test showed that the umbilical gray mottled soybean seed was positive for BPMV test, and the test of BPMV of umbilical black mottled soybean seed was negative.