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目的观察耳聋左慈丸对庆大霉素所致大鼠肾毒性和耳毒性的防治作用。方法将21只健康雄性Wistar大鼠随机分为对照组、庆大霉素组、中药防治组,每组7只。庆大霉素组和中药防治组均肌肉注射庆大霉素(80μg·g-1·d-1),每日2次,持续14天;中药防治组同时灌胃给予耳聋左慈丸(6 g·kg-1·d-1),每日1次,持续14天。对照组通过肌肉注射和灌胃给予等剂量的0.9%NaCl溶液,持续14天。采用听性脑干反应(ABR)检测听力水平,测定肾系数、血肌酐(Scr)和血尿素氮(BUN),光镜下观察肾组织的形态学改变。结果与对照组比较,庆大霉素组大鼠各频率ABR值显著增高(P<0.01),肾系数、血肌酐、血尿素氮升高(P<0.01)。与庆大霉素组比较,中药防治组大鼠各频率ABR值显著降低(P<0.01),肾系数显著下降(P<0.01),血肌酐、血尿素氮下降(P<0.05)。光镜下显示,庆大霉素组大鼠肾小管扩张、肾小管上皮细胞水肿、肾小管腔内有蛋白管型和细胞管型,而中药防治组肾小管结构基本正常。结论耳聋左慈丸可同时减缓庆大霉素引起的肾毒性和耳毒性。
Objective To observe the preventive and therapeutic effects of deafness Zuo Ci pill on gentamicin-induced nephrotoxicity and ototoxicity in rats. Methods Twenty-one healthy male Wistar rats were randomly divided into control group, gentamicin group and traditional Chinese medicine prevention and treatment group, with 7 rats in each group. Gentamycin group and traditional Chinese medicine prevention and treatment group were intramuscular injection of gentamicin (80μg · g-1 · d-1) twice daily for 14 days; Chinese medicine prevention and treatment group also given deafness Zuo Ci pills (6 g · kg-1 · d-1) once daily for 14 days. The control group was given an equal dose of 0.9% NaCl solution by intramuscular injection and gavage for 14 days. The auditory brainstem response (ABR) was used to detect the hearing level, and the renal coefficient, serum creatinine (Scr) and blood urea nitrogen (BUN) were measured. The morphological changes of kidney were observed under light microscope. Results Compared with the control group, ABR of gentamicin group was significantly increased (P <0.01), renal coefficient, serum creatinine and blood urea nitrogen increased (P <0.01). Compared with gentamicin group, ABR of rats in TCM group were significantly decreased (P <0.01), renal coefficient decreased significantly (P <0.01), serum creatinine and blood urea nitrogen decreased (P <0.05). Light microscopy showed that the rats in gentamicin group had dilated tubule, edema of renal tubular epithelial cells, protein tube type and cell type in renal tubular cavity, whereas those in traditional Chinese medicine group were basically normal. Conclusion Deafness Zuo Ci pill can simultaneously reduce gentamicin-induced nephrotoxicity and ototoxicity.