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建立D-(+)-葡萄糖酸δ-内酯–硼酸络合酸作为手性选择剂分离14种氨基醇类手性药物的非水毛细管电泳(NACE)新方法。实验考察了D-(+)-葡萄糖酸δ-内酯浓度、硼酸浓度、三乙胺浓度以及毛细管温度对14种氨基醇类手性药物分离效果的影响。确定了最佳手性分离条件为:未涂层熔融石英毛细管柱(55 cm×50μm ID,有效长度45 cm);背景缓冲液组成为200 mmol·L~(-1) D-(+)-葡萄糖酸δ-内酯、80 mmol·L~(-1)硼酸、57.4 mmol·L~(-1)三乙胺的甲醇溶液;进样压力2.9 psi,时间2 s;毛细管温度25±0.2℃;运行电压+15 k V;检测波长214 nm。在最佳分离条件下,大部分手性药物获得了良好的分离。本文为多羟基化合物–硼酸络合酸新型手性选择剂的原位合成及其在非水毛细管电泳中分离手性药物的应用奠定了基础。
A New Method of Nonaqueous Capillary Electrophoresis (NACE) for Separation of 14 Aminoalcoholic Chiral Drugs Using D - (+) - Gluconic Acid δ-Lactone-Boric Acid as Chiral Selector. The effect of D - (+) - gluconic acid δ-lactone concentration, boric acid concentration, triethylamine concentration and capillary temperature on the separation of 14 aminoalcohol chiral drugs was investigated experimentally. The optimum conditions of chiral separation were determined as follows: uncoated fused silica capillary column (55 cm × 50 μm ID, effective length 45 cm); the background buffer consisted of 200 mmol·L -1 D - (+) - Β-lactone gluconate, 80 mmol·L -1 boric acid and 57.4 mmol·L -1 triethylamine in methanol. The injection pressure was 2.9 psi and the time was 2 s. The capillary temperature was 25 ± 0.2 ℃ Operating voltage +15 kV; detection wavelength 214 nm. Under the best separation conditions, most of the chiral drugs get a good separation. In this paper, the synthesis of novel chiral selectors of polyhydroxy compound-boric acid complexation acid and its application in the separation of chiral drugs in non-aqueous capillary electrophoresis have been established.